Measurement

Part:BBa_J119138:Design

Designed by: Claire Shinneman, Paul Frederick   Group: Eckdahl_Lab   (2013-01-13)


Device for Testing New Promoters via Golden Gate Assembly


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1614
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 1615
    Illegal PstI site found at 1629
    Illegal NotI site found at 7
    Illegal NotI site found at 1622
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1615
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1615
    Illegal PstI site found at 1629
    Illegal AgeI site found at 1487
    Illegal AgeI site found at 1599
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 105
    Illegal BsaI site found at 837
    Illegal BsaI.rc site found at 28


Design Notes

None


Source

GGA BsmBI assembly of PCR product from BBa_K119013 and BBa_J119136

References